The advantages of E. coli expression systems include low cost, high expression, ease of scale-up, and short turnaround time. Our more than 10 host strains collection and various expression vectors guarantee the selection of the most efficient expression system. We offer multiple fusion/affinity tags to increase the protein solubility, expression levels, and ease of purification. Our proprietary AIE technology allows high yields of toxic proteins. For insoluble proteins, we have developed an effective protein refolding system consisting of 20 refolding buffers optimized with 9 parameters. If customers require large amounts of protein, we can also produce and purify from cultures as large as 35-500 liters. The E. coli system offers the metabolic labeling with stable isotopes.
E.coli Expression Flowchart
Service Name | Service Content | Timeline | |
---|---|---|---|
Plasmid Construction |
|
|
2 Weeks |
Protein Expression Test |
|
|
3 Weeks |
Large-Scale Fermentation & Purification |
|
3-5 Weeks |