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Acetyl-Histone H3-K36 Rabbit pAb (A16077)

Datasheet

ABclonal:Western blot - Acetyl-Histone H3-K36 Rabbit pAb (A16077)
ABclonal:Immunofluorescence - Acetyl-Histone H3-K36 Rabbit pAb (A16077)
ABclonal:Immunofluorescence - Acetyl-Histone H3-K36 Rabbit pAb (A16077)

概要

製品名Acetyl-Histone H3-K36 Rabbit pAb
品番A16077
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
免疫原A synthetic acetylated peptide around K36 of human Histone H3 (NP_003484.1).
配列GGVKK
遺伝子ID8290
Swiss protQ16695
別名H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW15kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:2000
IF 1:50 - 1:100
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunofluorescence    
陽性サンプルHeLa, NIH/3T3
細胞内局在性Chromosome, Nucleus
ABclonal:Western blot - Acetyl-Histone H3-K36 Rabbit pAb (A16077) }

Western blot - Acetyl-Histone H3-K36 Rabbit pAb (A16077)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K36 antibody (A16077) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K36 Rabbit pAb (A16077) }

Immunofluorescence - Acetyl-Histone H3-K36 Rabbit pAb (A16077)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K36 antibody (A16077) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K36 Rabbit pAb (A16077) }

Immunofluorescence - Acetyl-Histone H3-K36 Rabbit pAb (A16077)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K36 antibody (A16077) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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