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BID Rabbit pAb (A0210)

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ABclonal:Western blot - BID Rabbit pAb (A0210)
ABclonal:Immunofluorescence - BID Rabbit pAb (A0210)
ABclonal:Immunofluorescence - BID Rabbit pAb (A0210)
ABclonal:Immunofluorescence - BID Rabbit pAb (A0210)
Review
ABclonal: review for BID Rabbit pAb(A0210)

概要

製品名BID Rabbit pAb
品番A0210
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human BID (NP_001187.1).
配列MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATA
遺伝子ID637
Swiss protP55957
別名BID; FP497
Calculated MW11kDa/14kDa/21kDa/26kDa
Observed MW22kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:2000
IF 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunofluorescence    
陽性サンプルHL-60
細胞内局在性Cytoplasm, Mitochondrion membrane, Mitochondrion membrane
ユーザー検証応用

(Human fibrosarcoma cells)

WB(Bos taurus)

ABclonal:Western blot - BID Rabbit pAb (A0210) }

Western blot - BID Rabbit pAb (A0210)

Western blot analysis of extracts of HL-60 cells, using BID Antibody (A0210).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Immunofluorescence - BID Rabbit pAb (A0210) }

Immunofluorescence - BID Rabbit pAb (A0210)

Immunofluorescence analysis of C6 cells using BID antibody (A0210) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BID Rabbit pAb (A0210) }

Immunofluorescence - BID Rabbit pAb (A0210)

Immunofluorescence analysis of NIH/3T3 cells using BID antibody (A0210) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BID Rabbit pAb (A0210) }

Immunofluorescence - BID Rabbit pAb (A0210)

Immunofluorescence analysis of U2OS cells using BID antibody (A0210) at dilution of 1:100. Blue: DAPI for nuclear staining.

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