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Bcl-2 Rabbit pAb (A0208)

レビュー (7)使用論文(101) Datasheet

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)

概要

製品名Bcl-2 Rabbit pAb
品番A0208
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
免疫原A synthetic peptide of human Bcl-2
配列Email for sequence
遺伝子ID596
Swiss protP10415
別名Bcl-2; PPP1R50; BCL2
Calculated MW22kDa/26kDa
Observed MW26KDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
陽性サンプル293T, THP-1, HeLa
細胞内局在性Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
ユーザー検証応用

(rat cells, Human skin T cell lymphoma, CAL27, Hut102, HCT116, B6, MCF-7,MD-MBA-231)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Bos taurus, Capra hircus, Ctenopharyngodon, Ctenopharyngodon idellus, Giardia duodenalis)

IHC(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Mus musculus)

IF(Homo sapiens)

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208) }

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A0208) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208) }

Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)

Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of BALB-3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208) }

Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)

Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (A0208). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A0208) at a dilition of 1:1000.

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