製品センター > 抗体製品 > ポリクローナル抗体

Bcl-2 Rabbit pAb (A2212)

レビュー (3)使用論文(5) Datasheet

ABclonal:Western blot - Bcl-2 Rabbit pAb (A2212)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A2212)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A2212)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A2212)

概要

製品名Bcl-2 Rabbit pAb
品番A2212
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
免疫原Recombinant protein of human Bcl-2
配列Email for sequence
遺伝子ID596
Swiss protP10415
別名Bcl-2; PPP1R50; BCL2
Calculated MW22kDa/26kDa
Observed MW26kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:1000
IHC 1:50 - 1:100
IP 1:20 - 1:50
FC 1:20 - 1:50
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunoprecipitation    Flow Cytometry    
陽性サンプルJurkat, U-937, MCF7, Mouse liver, Rat brain
細胞内局在性Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
ユーザー検証応用

(HK2, Mouse kidney, Human)

WB(Rattus norvegicus, Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus)

IF(Homo sapiens,Rattus norvegicus)

Co-IP(Homo sapiens)

ABclonal:Western blot - Bcl-2 Rabbit pAb (A2212) }

Western blot - Bcl-2 Rabbit pAb (A2212)

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A2212) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Submit an review for A2212

Please submit reviews to your technical sales specialist directly. Alternatively, email us at jp.market@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A2212 below and we will get back to you within one business day.

Publishing research using A2212? Please let us know so that we can cite the reference in this datasheet.

価格と注文
お問い合わせ