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CCNE1 Rabbit pAb (A14225)

使用論文(1) Datasheet

ABclonal:Western blot - CCNE1 Polyclonal Antibody (A14225)

概要

製品名CCNE1 Rabbit pAb
品番A14225
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human CCNE1 (NP_001229.1).
配列AASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
遺伝子ID898
Swiss protP24864
別名CCNE1; CCNE; pCCNE1; cyclin E1
Calculated MW47kDa
Observed MW50kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルMCF7
細胞内局在性Nucleus
ユーザー検証応用

WB(Homo sapiens)

ABclonal:Western blot - CCNE1 Polyclonal Antibody (A14225) }

Western blot - CCNE1 Polyclonal Antibody (A14225)

Western blot analysis of extracts of various cell lines, using CCNE1 antibody (A14225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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価格と注文

6月1日-7月31日 キャンペーン価格
35000円 /100 μL.

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