製品センター > 抗体製品 > ポリクローナル抗体

CD14 Rabbit pAb (A5737)

Datasheet

ABclonal:Western blot - CD14 Polyclonal Antibody (A5737)
ABclonal:Immunohistochemistry - CD14 Polyclonal Antibody (A5737)
ABclonal:Immunofluorescence - CD14 Polyclonal Antibody (A5737)
ABclonal:Immunofluorescence - CD14 Polyclonal Antibody (A5737)

概要

製品名CD14 Rabbit pAb
品番A5737
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD14 (NP_001167576.1).
配列TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNP
遺伝子ID929
Swiss protP08571
別名CD14
Calculated MW40kDa
Observed MW70kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunofluorescence    
陽性サンプルMouse lung, Mouse spleen, Mouse kidney
細胞内局在性Cell membrane, GPI-anchor, Lipid-anchor, Secreted
ABclonal:Western blot - CD14 Polyclonal Antibody (A5737) }

Western blot - CD14 Polyclonal Antibody (A5737)

Western blot analysis of extracts of various cell lines, using CD14 antibody (A5737) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - CD14 Polyclonal Antibody (A5737) }

Immunohistochemistry - CD14 Polyclonal Antibody (A5737)

Immunohistochemistry of paraffin-embedded human spleen using CD14 antibody (A5737) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD14 Polyclonal Antibody (A5737) }

Immunofluorescence - CD14 Polyclonal Antibody (A5737)

Immunofluorescence analysis of RAW264.7 cells using CD14 antibody (A5737) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD14 Polyclonal Antibody (A5737) }

Immunofluorescence - CD14 Polyclonal Antibody (A5737)

Immunofluorescence analysis of THP-1 cells using CD14 antibody (A5737) at dilution of 1:100. Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A5737 below and we will get back to you within one business day.

価格と注文
お問い合わせ