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CD19 Rabbit pAb (A2577)

使用論文(1) Datasheet

ABclonal:Western blot - CD19 Rabbit pAb (A2577)
ABclonal:Immunohistochemistry - CD19 Rabbit pAb (A2577)
ABclonal:Immunohistochemistry - CD19 Rabbit pAb (A2577)
ABclonal:Immunohistochemistry - CD19 Rabbit pAb (A2577)
ABclonal:Immunofluorescence - CD19 Rabbit pAb (A2577)

概要

製品名CD19 Rabbit pAb
品番A2577
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 351-556 of human CD19 (NP_001761.3).
配列VLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR
遺伝子ID930
Swiss protP15391
別名B4; CVID3; CD19
Calculated MW61kDa
Observed MW95kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunofluorescence    
陽性サンプルRaji
細胞内局在性Membrane, Single-pass type I membrane protein
ユーザー検証応用

FC(Homo sapiens)

ABclonal:Western blot - CD19 Rabbit pAb (A2577) }

Western blot - CD19 Rabbit pAb (A2577)

Western blot analysis of extracts of Raji cells, using CD19 Antibody (A2577) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD19 Rabbit pAb (A2577) }

Immunohistochemistry - CD19 Rabbit pAb (A2577)

Immunohistochemistry of paraffin-embedded rat spleen using CD19 antibody (A2577) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD19 Rabbit pAb (A2577) }

Immunohistochemistry - CD19 Rabbit pAb (A2577)

Immunohistochemistry of paraffin-embedded human vermiform appendix using CD19 antibody (A2577) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD19 Rabbit pAb (A2577) }

Immunohistochemistry - CD19 Rabbit pAb (A2577)

Immunohistochemistry of paraffin-embedded human appendix using CD19 antibody (A2577) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD19 Rabbit pAb (A2577) }

Immunofluorescence - CD19 Rabbit pAb (A2577)

Immunofluorescence analysis of mouse spleen using CD19 Rabbit pAb (A2577) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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価格と注文

2021年4月1日(木)~5月31日(月) キャンペーン期間 50μl 20% OFF

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