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DiMethyl-Histone H3-K9 Rabbit pAb (A2359)

レビュー (2)使用論文(10) Datasheet

ABclonal:Western blot - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)
ABclonal: - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)
ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)
Review
ABclonal: review for DiMethyl-Histone H3-K9 Rabbit pAb(A2359)
Review
ABclonal: review for DiMethyl-Histone H3-K9 Rabbit pAb(A2359)

概要

製品名DiMethyl-Histone H3-K9 Rabbit pAb
品番A2359
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
免疫原A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
配列Email for sequence
遺伝子ID8290
Swiss protQ16695
別名H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW18kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
ChIPseq 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
陽性サンプルHeLa
細胞内局在性Chromosome, Nucleus
ユーザー検証応用

(293FT,PC9, UBQ10)

WB(Homo sapiens, Mus musculus)

DB(Homo sapiens)

WB(Homo sapiens)

ChIP-qPCR(Homo sapiens)

ABclonal:Western blot - DiMethyl-Histone H3-K9 Rabbit pAb (A2359) }

Western blot - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K9 antibody (A2359).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal: - DiMethyl-Histone H3-K9 Rabbit pAb (A2359) }

- DiMethyl-Histone H3-K9 Rabbit pAb (A2359)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K9 antibody (A2359).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 Rabbit pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)

Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K9 Rabbit pAb (A2359) }

Immunofluorescence - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K9 antibody (A2359). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K9 Rabbit pAb (A2359) }

Chromatin Immunoprecipitation - DiMethyl-Histone H3-K9 Rabbit pAb (A2359)

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K9 antibody (A2359) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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価格と注文

6月1日-7月31日 キャンペーン価格
50000円 /100 μL.

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