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GAPDH Mouse mAb (AC033)

使用論文(43) Datasheet

ABclonal:Western blot - GAPDH Mouse mAb (AC033)
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033)
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033)
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033)
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033)
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033)
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033)
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC033)

概要

製品名GAPDH Mouse mAb
品番AC033
由来種Mouse
精製方式Affinity purification
アイソタイプIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
免疫原Recombinant protein of human GAPDH
配列Email for sequence
遺伝子ID2597
Swiss protP04406
別名G3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36KDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:50000 - 1:200000
IHC 1:50 - 1:200
IF 1:50 - 1:200
抗体希釈法Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunofluorescence    
陽性サンプルHeLa, HepG2, 293T, Mouse liver, Mouse kidney, Mouse spleen, Rat liver, Rat kidney
細胞内局在性Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
ユーザー検証応用

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

IHC(Homo sapiens)

IP(Mus musculus)

IF(Rattus norvegicus)

DB(Homo sapiens)

ABclonal:Western blot - GAPDH Mouse mAb (AC033) }

Western blot - GAPDH Mouse mAb (AC033)

Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (AC033)

Immunohistochemistry of paraffin-embedded rat spleen using GAPDH antibody (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (AC033)

Immunohistochemistry of paraffin-embedded rat heart using GAPDH antibody (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (AC033)

Immunohistochemistry of paraffin-embedded human tonsil using GAPDH antibody (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (AC033)

Immunohistochemistry of paraffin-embedded human lung cancer using GAPDH antibody (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (AC033)

Immunohistochemistry of paraffin-embedded human colon using GAPDH antibody (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (AC033)

Immunohistochemistry of paraffin-embedded mouse spinal cord using GAPDH antibody (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC033) }

Immunofluorescence - GAPDH Mouse mAb (AC033)

Immunofluorescence analysis of PC-12 cells using GAPDH antibody (AC033) at dilution of 1:200. Blue: DAPI for nuclear staining.

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