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GRO alpha Rabbit pAb (A5802)

使用論文(1) Datasheet

ABclonal:Western blot - GRO alpha Polyclonal Antibody (A5802)

概要

製品名GRO alpha Rabbit pAb
品番A5802
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human GRO alpha (NP_001502.1).
配列MARAALSAAPSNPRLLRVALLLLLLVAAGRRAAGASVATELRCQCLQTLQGIHPKNIQSVNVKSPGPHCAQTEVIATLKNGRKACLNPASPIVKKIIEKMLNSDKSN
遺伝子ID2919
Swiss protP09341
別名CXCL1; FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1; GRO alpha
Calculated MW11kDa
Observed MW11kDa
反応動物種Human
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:2000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルDU145
細胞内局在性Secreted
ユーザー検証応用

WB(Homo sapiens,Mus musculus)

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - GRO alpha Polyclonal Antibody (A5802) }

Western blot - GRO alpha Polyclonal Antibody (A5802)

Western blot analysis of extracts of DU145 cells, using GRO alpha antibody (A5802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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