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HIF1A Polyclonal Antibody (A6265)

レビュー (3)使用論文(2) Datasheet

ABclonal:Western blot - HIF1A Polyclonal Antibody (A6265)
ABclonal:Western blot - HIF1A Polyclonal Antibody (A6265)
Review
ABclonal: review for HIF1A Polyclonal Antibody(A6265)
Review
ABclonal: review for HIF1A Polyclonal Antibody(A6265)
Review
ABclonal: review for HIF1A Polyclonal Antibody(A6265)

概要

製品名HIF1A Polyclonal Antibody
品番A6265
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
免疫原A synthetic peptide of human HIF1A
配列Email for sequence
遺伝子ID3091
Swiss protQ16665
別名HIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78
Calculated MW82kDa/92kDa/95kDa
Observed MW120kDa
反応動物種Human
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:1000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルHeLa, Raji
細胞内局在性Cytoplasm, Nucleus, Nucleus speckle
ユーザー検証応用

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WB(Human, Zebrafish)

ABclonal:Western blot - HIF1A Polyclonal Antibody (A6265) }

Western blot - HIF1A Polyclonal Antibody (A6265)

Western blot analysis of extracts of HeLa cells, using HIF1A antibody (A6265) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - HIF1A Polyclonal Antibody (A6265) }

Western blot - HIF1A Polyclonal Antibody (A6265)

Western blot analysis of extracts of Raji cells, using HIF1A antibody (A6265) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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