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HIF1α Rabbit pAb (A11945)

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ABclonal:Western blot - HIF1α Rabbit pAb (A11945)
Review
ABclonal: review for HIF1α Rabbit pAb(A11945)

概要

製品名HIF1α Rabbit pAb
品番A11945
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 70-260 of human HIF1α (NP_851397.1).
配列RKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERIT
遺伝子ID3091
Swiss protQ16665
別名HIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78
Calculated MW82kDa/92kDa/95kDa
Observed MW120KDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルHeLa
細胞内局在性Cytoplasm, Nucleus, Nucleus speckle
ユーザー検証応用

(N9 cells)

WB(Homo sapiens, Gallus gallus, Rattus norvegicus, Mus musculus)

IF(Homo sapiens)

Co-IP(Homo sapiens)

IHC(Gallus gallus)

ABclonal:Western blot - HIF1α Rabbit pAb (A11945) }

Western blot - HIF1α Rabbit pAb (A11945)

Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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価格と注文

2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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