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IL10 Rabbit pAb (A2171)

レビュー (1)使用論文(7) Datasheet

ABclonal:Western blot - IL10 Polyclonal Antibody (A2171)
ABclonal:Immunohistochemistry - IL10 Polyclonal Antibody (A2171)
Review
ABclonal: review for IL10 Rabbit pAb(A2171)

概要

製品名IL10 Rabbit pAb
品番A2171
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1).
配列SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
遺伝子ID3586
Swiss protP22301
別名CSIF; GVHDS; IL-10; IL10A; TGIF; IL10
Calculated MW20kDa
Observed MW22kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:100
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    
陽性サンプルJurkat
細胞内局在性Secreted
ユーザー検証応用

(Mouse embryonal fibroblast cell)

WB(Rattus norvegicus, Mus musculus, Cynoglossus semilaevis, Homo sapiens)

ABclonal:Western blot - IL10 Polyclonal Antibody (A2171) }

Western blot - IL10 Polyclonal Antibody (A2171)

Western blot analysis of extracts of Jurkat cells, using IL10 antibody (A2171) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IL10 Polyclonal Antibody (A2171) }

Immunohistochemistry - IL10 Polyclonal Antibody (A2171)

Immunohistochemistry of paraffin-embedded mouse spleen using IL10 antibody (A2171) at dilution of 1:200 (40x lens).

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価格と注文

2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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