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IL17F Rabbit pAb (A6487)

Datasheet

ABclonal:Western blot - IL17F Polyclonal Antibody (A6487)
ABclonal:Immunofluorescence - IL17F Polyclonal Antibody (A6487)

概要

製品名IL17F Rabbit pAb
品番A6487
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 31-163 of human IL17F (NP_443104.1).
配列RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ
遺伝子ID112744
Swiss protQ96PD4
別名IL17F; CANDF6; IL-17F; ML-1; ML1
Calculated MW18kDa
Observed MW18kDa
反応動物種Human, Mouse
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:2000
IF 1:50 - 1:100
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunofluorescence    
陽性サンプルK-562, U-87MG
細胞内局在性Secreted
ABclonal:Western blot - IL17F Polyclonal Antibody (A6487) }

Western blot - IL17F Polyclonal Antibody (A6487)

Western blot analysis of extracts of various cell lines, using IL17F antibody (A6487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - IL17F Polyclonal Antibody (A6487) }

Immunofluorescence - IL17F Polyclonal Antibody (A6487)

Immunofluorescence analysis of MCF7 cells using IL17F antibody (A6487). Blue: DAPI for nuclear staining.

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