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IL6 Rabbit pAb (A0286)

レビュー (4)使用論文(18) Datasheet

ABclonal:Western blot - IL6 Rabbit pAb (A0286)
ABclonal:Immunohistochemistry - IL6 Rabbit pAb (A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)
Review
ABclonal: review for IL6 Rabbit pAb(A0286)

概要

製品名IL6 Rabbit pAb
品番A0286
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6 (NP_000591.1).
配列VPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
遺伝子ID3569
Swiss protP05231
別名IL6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Calculated MW23kDa
Observed MW27kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    
陽性サンプルHeLa, Mouse heart, Rat heart
細胞内局在性Secreted
ユーザー検証応用

(Human colon cancer cell, Mouse skeletal muscle, Mouse embryonal fibroblast cell)

IHC(Homo sapiens, Mus musculus)

WB(Mus musculus, Rattus norvegicus, Oryctolagus cuniculus, Homo sapiens, Cynoglossus semilaevis)

ELISA(Mus musculus)

WB(Mus musculus)

IHC(Mus musculus)

IF(Homo sapiens,Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - IL6 Rabbit pAb (A0286) }

Western blot - IL6 Rabbit pAb (A0286)

Western blot analysis of extracts of various cell lines, using IL6 antibody (A0286) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - IL6 Rabbit pAb (A0286) }

Immunohistochemistry - IL6 Rabbit pAb (A0286)

Immunohistochemistry of paraffin-embedded mouse spleen using IL6 antibody (A0286) at dilution of 1:50 (40x lens).

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価格と注文

2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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