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KIR2DS4 Rabbit pAb (A1779)

Datasheet

ABclonal:Western blot - KIR2DS4 Polyclonal Antibody (A1779)
ABclonal:Immunofluorescence - KIR2DS4 Polyclonal Antibody (A1779)

概要

製品名KIR2DS4 Rabbit pAb
品番A1779
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several 'framework' genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 22-245 of human KIR2DS4 (NP_036446.3).
配列QEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLH
遺伝子ID3809
Swiss protP43632
別名KIR2DS4; CD158I; KIR-2DS4; KIR1D; KIR412; KKA3; NKAT-8; NKAT8
Calculated MW33kDa
Observed MW35kDa
反応動物種Human
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IF 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunofluorescence    
陽性サンプル293T
細胞内局在性Cell membrane, Single-pass type I membrane protein
ABclonal:Western blot - KIR2DS4 Polyclonal Antibody (A1779) }

Western blot - KIR2DS4 Polyclonal Antibody (A1779)

Western blot analysis of extracts of 293T cells, using KIR2DS4 antibody (A1779) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - KIR2DS4 Polyclonal Antibody (A1779) }

Immunofluorescence - KIR2DS4 Polyclonal Antibody (A1779)

Immunofluorescence analysis of U2OS cells using KIR2DS4 antibody (A1779) at dilution of 1:100. Blue: DAPI for nuclear staining.

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