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[KO Validated] IL18 Rabbit pAb (A1115)

レビュー (2)使用論文(11) Datasheet

ABclonal:Western blot - [KO Validated] IL18 Rabbit pAb (A1115)
ABclonal:Western blot - [KO Validated] IL18 Rabbit pAb (A1115)
ABclonal:Immunoprecipitation - [KO Validated] IL18 Rabbit pAb (A1115)
Review
ABclonal: review for [KO Validated] IL18 Rabbit pAb(A1115)
Review
ABclonal: review for [KO Validated] IL18 Rabbit pAb(A1115)

概要

製品名[KO Validated] IL18 Rabbit pAb
品番A1115
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).
配列YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
遺伝子ID3606
Swiss protQ14116
別名IL18; IGIF; IL-18; IL-1g; IL1F4
Calculated MW21kDa/22kDa
Observed MW23kDa
反応動物種Human, Mouse
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IP 1:50 - 1:100
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunoprecipitation    
陽性サンプルHeLa, Mouse lung
細胞内局在性Secreted
ユーザー検証応用

(HUVEC cells, N9 cells)

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - [KO Validated] IL18 Rabbit pAb (A1115) }

Western blot - [KO Validated] IL18 Rabbit pAb (A1115)

Western blot analysis of extracts of various cell lines, using IL18 antibody (A1115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Western blot - [KO Validated] IL18 Rabbit pAb (A1115) }

Western blot - [KO Validated] IL18 Rabbit pAb (A1115)

Western blot analysis of extracts from normal (control) and IL18 knockout (KO) HeLa cells, using IL18 antibody (A1115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - [KO Validated] IL18 Rabbit pAb (A1115) }

Immunoprecipitation - [KO Validated] IL18 Rabbit pAb (A1115)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IL18 antibody (A1115). Western blot was performed from the immunoprecipitate using IL18 antibody (A1115) at a dilition of 1:1000.

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2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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