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PEDF Rabbit pAb (A1673)

Datasheet

ABclonal:Western blot - PEDF Polyclonal Antibody (A1673)

概要

製品名PEDF Rabbit pAb
品番A1673
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PEDF (NP_002606.3).
配列MQALVLLLCIGALLGHSSCQNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIP
遺伝子ID5176
Swiss protP36955
別名SERPINF1; EPC-1; OI12; OI6; PEDF; PIG35
Calculated MW46kDa
Observed MW46kDa
反応動物種Human, Mouse
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
推奨希釈比WB 1:500 - 1:2000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルA375, Mouse kidney
細胞内局在性Melanosome, Secreted
ABclonal:Western blot - PEDF Polyclonal Antibody (A1673) }

Western blot - PEDF Polyclonal Antibody (A1673)

Western blot analysis of extracts of various cell lines, using PEDF antibody (A1673) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

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