製品センター > 抗体製品 > リン酸化抗体

Phospho-AKT1-S473 Rabbit mAb (AP0637)

レビュー (2)使用論文(0) Datasheet

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit mAb (AP0637)
ABclonal:Western blot - Phospho-AKT1-S473 Rabbit mAb (AP0637)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit mAb(AP0637)
Review
ABclonal: review for Phospho-AKT1-S473 Rabbit mAb(AP0637)

概要

製品名Phospho-AKT1-S473 Rabbit mAb
品番AP0637
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
免疫原A phospho specific peptide corresponding to residues surrounding S473 of human AKT1
配列Email for sequence
遺伝子ID207
Swiss protP31749
別名AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW48kDa/55kDa
Observed MW60KDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルJurkat, NIH/3T3, C6
細胞内局在性Cell membrane, Cytoplasm, Nucleus
ユーザー検証応用

(Human lung cancer cells)

ABclonal:Western blot - Phospho-AKT1-S473 Rabbit mAb (AP0637) }

Western blot - Phospho-AKT1-S473 Rabbit mAb (AP0637)

Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473 pAb (AP0637) at 1:1000 dilution.Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-AKT1-S473 Rabbit mAb (AP0637) }

Western blot - Phospho-AKT1-S473 Rabbit mAb (AP0637)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-AKT1-S473 pAb (AP0637) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Submit an review for AP0637

Please submit reviews to your technical sales specialist directly. Alternatively, email us at jp.market@abclonal.com

Reviews List

Inquire About This Product

Submit your question about AP0637 below and we will get back to you within one business day.

価格と注文
お問い合わせ