Western blot - Phospho-Tau-Ser198/Ser199/Ser202 Rabbit pAb (AP1096)
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Tau-Ser198/Ser199/Ser202 pAb (AP1096) at 1:1000 dilution or Tau antibody (A0002).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.