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TGFB1 Rabbit pAb (A2124)

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ABclonal:Western blot - TGFB1 Rabbit pAb (A2124)
Review
ABclonal: review for TGFB1 Rabbit pAb(A2124)

概要

製品名TGFB1 Rabbit pAb
品番A2124
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 30-280 of human TGFB1 (NP_000651.3).
配列LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRAL
遺伝子ID7040
Swiss protP01137
別名TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-β
Calculated MW44kDa
Observed MW44kDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    
陽性サンプルA-549, Rat heart
細胞内局在性Secreted, extracellular matrix, extracellular space
ユーザー検証応用

(Rat heart)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa)

IHC(Mus musculus)

ABclonal:Western blot - TGFB1 Rabbit pAb (A2124) }

Western blot - TGFB1 Rabbit pAb (A2124)

Western blot analysis of extracts of various cell lines, using TGFB1 antibody (A2124) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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価格と注文

2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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