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VEGFA Rabbit pAb (A5708)

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ABclonal:Western blot - VEGFA Rabbit pAb (A5708)
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ABclonal: review for VEGFA Rabbit pAb(A5708)
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ABclonal: review for VEGFA Rabbit pAb(A5708)

概要

製品名VEGFA Rabbit pAb
品番A5708
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human VEGFA (NP_001165099.1).
配列MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR
遺伝子ID7422
Swiss protP15692
別名VEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A
Calculated MW15-27kDa/34-45kDa
Observed MW38kDa
反応動物種Human, Mouse
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    
陽性サンプルBT-474, Mouse heart, Mouse kidney, Mouse gastrocnemius muscle
細胞内局在性Secreted
ユーザー検証応用

(H460, HUVEC cells)

WB(Homo sapiens, Gallus gallus, Rattus norvegicus)

IHC(Gallus gallus)

IHC(Homo sapiens)

IF(Gallus gallus)

ABclonal:Western blot - VEGFA Rabbit pAb (A5708) }

Western blot - VEGFA Rabbit pAb (A5708)

Western blot analysis of extracts of various cell lines, using VEGFA antibody (A5708) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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価格と注文

2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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