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VEGF Rabbit pAb (A12303)

レビュー (1)使用論文(8) Datasheet

ABclonal:Western blot - VEGF Rabbit pAb (A12303)
ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303)
ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303)
ABclonal:Immunofluorescence - VEGF Rabbit pAb (A12303)
Review
ABclonal: review for VEGF Rabbit pAb(A12303)

概要

製品名VEGF Rabbit pAb
品番A12303
由来種Rabbit
精製方式Affinity purification
アイソタイプIgG
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 27-126AA of human VEGF (NP_001165099.1).
配列APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN
遺伝子ID7422
Swiss protP15692
別名VEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A
Calculated MW15-27kDa/34-45kDa
Observed MW16KDa/34KDa/45KDa
反応動物種Human, Mouse, Rat
検証応用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
推奨希釈比WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
保存用バッファーStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
検査方法Western blotting    Immunohistochemistry    Immunofluorescence    
陽性サンプル293T, A-549, HeLa, Mouse liver
細胞内局在性Secreted
ユーザー検証応用

(Mouse bone tissue)

WB(Homo sapiens, Mus musculus, Neovison vison, Oryctolagus cuniculus)

IHC(Mus musculus)

IF(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - VEGF Rabbit pAb (A12303) }

Western blot - VEGF Rabbit pAb (A12303)

Western blot analysis of extracts of various cell lines, using VEGF antibody (A12303) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303) }

Immunohistochemistry - VEGF Rabbit pAb (A12303)

Immunohistochemistry of paraffin-embedded Human esophagus cancer using VEGF antibody (A12303) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - VEGF Rabbit pAb (A12303) }

Immunohistochemistry - VEGF Rabbit pAb (A12303)

Immunohistochemistry of paraffin-embedded Human lung cancer using VEGF antibody (A12303) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - VEGF Rabbit pAb (A12303) }

Immunofluorescence - VEGF Rabbit pAb (A12303)

Immunofluorescence analysis of HUVEC cells using VEGFA antibody (A12303) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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価格と注文

2020年12月1日~2021年1月29日 キャンペーン期間 100μl 20% OFF

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