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Recombinant Human CLPS/Colipase Protein (RP00125)

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概要

製品名Recombinant Human CLPS/Colipase Protein
品番RP00125
DescriptionRecombinant Human CLPS/Colipase Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Gln112) of human CLPS/Colipase (Accession #NP_001823.1) fused with a 6×His tag at the C-terminus.
Swiss portP04118
遺伝子ID1208
由来種Human
配列 MEKILILLLVALSVAYAAPGPRGIIINLENGELCMNSAQCKSNCCQHSSALGLARCTSMASENSECSVKTLYGIYYKCPCERGLTCEGDKTIVGSITNTNFGICHDAGRSKQ
The protein is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene.
Expressed HostHEK293 cells
Tag6×His tag at the C-terminus
Purity> 90% by SDS-PAGE.
Bio-ActivityMeasured by its ability to block I-309-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR8. The ED50 for this effect is 15-75 ng/mL in the presence of 20 ng/mL rhI-309.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
StorageStore the lyophilized protein at -20°C to -80 °C for long term. |After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
ReconstitutionReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

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