|製品名||Recombinant Mouse PD-1/PDCD1/CD279 Protein|
|Description||Recombinant Mouse PD-1/PDCD1/CD279 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Leu25-Gln167) of mouse PD-1/PDCD1/CD279 (Accession #Q02242) fused with a 6×His tag at the C-terminus.|
Programmed Death-1 (PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member ofCD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an importantimmunosuppressive molecule. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptortyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM) formed bytwo tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymusand shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1 (PD-1) with itsligands, programmed death ligand B7H1 (PD-L1) and B7DC (PD-L2), was found to regulate T-cell activation andtolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 geneknocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitorymolecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for theimmunologic therapy of carcinoma, infection, autoimmune diseases as well as organ transplantation.
|Synonym||Programmed cell death protein 1, PD-1, CD279, Pdcd1, mPD-1|
|Expressed Host||Human cells|
|Tag||6×His tag at the C-terminus|
|Purity||> 95% by SDS-PAGE.|
|Endotoxin||< 1 EU/μg of the protein by LAL method. |
|Formulation||Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.|
|Storage||Store the lyophilized protein at -20°C to -80 °C for long term.|After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|
|Reconstitution||Reconstitute to a concentration of 0.1-0.5 mg/mL in 1X PBS.|
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