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ABclonal First Strand Synthesis Module (RK20353)

Datasheet

Catalog No.: RK20353
Sequencer: Illumina

ABclonal First Strand Synthesis Module (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product.

Product Description

ABclonal First Strand Synthesis Module (Stranded) (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Reagent in this module does not contain Actinomycin D and can be used with ABclonal Second Strand Synthesis Module (RK20346) to make RNA libraries. In addition, Frag/Elute Buffer employs Mg2+ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg2+ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.
Component24RXN (RK20353M)96RXN (RK20353L)
2×Frag/Elute Buffer132μL528 μL
RT Reagent192μL768 μL
First Strand Synthesis Enzyme Mix48μL192 μL
Stored at -20°C.
Poly(A)RNA, prepared using ABclonal Poly(A) mRNA Purification Module (RK20341)
rRNA depleted RNA, prepared using ABclonal rRNA Depletion Module (H/M/R) (RK20348)
200μL PCR tube
Thermocycler
Pipette

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