ABclonal First Strand Synthesis Module (RK20353)

ABclonal First Strand Synthesis Module (Stranded) (RK20353) is designed for RNA fragmentation and first strand cDNA synthesis in RNA-seq library construction. The second strand cDNA can be directly synthesized without purifying the first strand cDNA product. The RT Reagent in this module does not contain Actinomycin D and can be used with ABclonal Second Strand Synthesis Module (RK20346) to make RNA libraries. In addition, Frag/Elute Buffer employs Mg²⁺ at high temperature conditions to fragment the RNA. Therefore, the RNA sample cannot contain metal ions such as Mg²⁺ or a metal ion chelating agent such as EDTA, which may affect the fragmentation size of RNA.