Catalog No.: RK20302
Sequencer: Illumina
The ABclonal mRNA-seq Lib Prep Kit for IlluminaR comprises four processing modules: Poly(A) mRNA Purification, First Strand Synthesis, Second cDNA Synthesis and DNA Library Preparation.
| Box | Module Name | Tube Color & Name | 24 Reactions (RK20302M) | 96 Reactions (RK20302L) |
| Box-1 | Poly(A) mRNA Purification Module (RK20341) | Oligo d(T)25 Capture Beads | 480 μl | 1920 μl |
| mRNA Binding Buffer | 12 ml | 48 ml | ||
| Washing Buffer | 19.2 ml | 76.8 ml | ||
| Tris Buffer | 1.2 ml | 4.8 ml | ||
| Box-2 | First Strand Synthesis Module (RK20353) | 2X Frag/Elute Buffer | 144 μl | 576 μl |
| RT Reagent | 192 μl | 768 μl | ||
| First Strand Synthesis Enzyme Mix | 48 μl | 192 μl | ||
| Second Strand Synthesis Module (RK20346) | Second Strand Synthesis Reaction Buffer | 192 μl | 768 μl | |
| Second Strand Synthesis Enzyme Mix | 96 μl | 384 μl | ||
| Nuclease-free Water | 2 ml | 8 ml | ||
| DNA Lib Prep Module (RK20347) | End Prep Buffer | 240 μl | 960 μl | |
| End Prep Enzyme Mix | 72 μl | 288 μl | ||
| Ligation Buffer | 396 μl | 1584 μl | ||
| Ligase Mix | 72 μl | 288 μl | ||
| 2X PCR Mix | 600 μl | 2×1200 μl | ||
| Low-EDTA TE | 2.5 ml | 10 ml | ||
| Box-3 | RNA Adapter Module 24 Index | RNA Truncated Adapter | 60 μl | 240 μl |
| RNA Universal Primer | 60 μl | 240 μl | ||
| RNA Index Primer | 2.5 μl* | 10 μl* |
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