The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina platforms. The entire protocol can be completed in 2 hours.
The Rapid DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina (R) platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The Low-EDTA TE buffer can be stored at room temperature or 4°C. All other components should be stored at -20°C. The shelf lives of all reagents are one year when stored properly.
Mechanical or enzymatic methods for DNA fragmentation Multiplex adapters (cat. no. RK20282, RK20283, RK20284, RK20285, RK20292, RK20293, RK20294, RK20295) compatible with Illumina(R) platforms 100% ethanol (ACS grade) Nuclease-free water PCR strip tubes or plates Magnetic stand Thermocycler AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880) Pipettes and multichannel pipettes Aerosol resistant pipette tips Microcentrifuge Vortex mixer Agilent Bioanalyzer or comparable method to assess the quality of DNA library
A Novel Polysaccharide Depolymerase Encoded by the Phage SH-KP152226 Confers Specific Activity Against Multidrug-Resistant Klebsiella pneumoniae via Biofilm Degradation.
Publishing research using RK20200? Please let us know so that we can cite the reference in this datasheet.
